When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

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Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...

The detection of viable cells is pivotal in microbiology, environmental monitoring and food safety, where distinguishing live from dead organisms can significantly influence risk assessments and ...